Preparation method and application of electrochemical micro-fluidic sensing chip

The invention provides a preparation method and the application of an electrochemical micro-fluidic sensing chip. The preparation method comprises the following steps: directly coating an improved glass solution on a commercial standard printed electrode; and performing vacuum plasma treatment on a PDMS (Polydimethylsiloxane) chip with pre-designed pipelines and the printed electrode coated with the glass solution together, and directly bonding the PDMS chip on the commercial standard printed electrode to form a novel electrochemical microfluidic sensing platform. A sensor provided by the invention can perform ultrasensitive detection on various sample analytes in a biological fluid sample, taking the detection of a prostate cancer marker PSA (Prostate-specific Antigen) in human serum as an example, a coulomb amperometry is used for detection, and a result shows that the detection sensitivity can reach 0.84 pg/mL which is improved by two magnitudes than the standardized clinical testing requirement of 0.1 ng/mL, so that the sensor has superhigh detection sensitivity and accuracy, which are higher than those of other electrochemical detection devices, is convenient in operation, and can integrates sample processing, separation and the like on one micro electrochemical microfluidic sensing chip.