Scientific Evaluation of Antioxidant and Anti Cancer Activity of Kanchanara Guggulu Vati by Invitro Methods

AIM AND OBJECTIVES OF THE STUDY: Preliminary phytochemical analysis of the polyherbal formulation “Kanchanara guggulu vati”. To evaluate the antioxidant activity (DPPH method) of polyherbal formulation “Kanchanara guggulu vati”. To evaluate the IC50 concentration of methanolic crude extract of “Kanchanara guggulu vati” by in- vitro cytotoxicity method (MTT assay). To isolate the flavonoid fraction from polyherbal formulation “Kanchanara guggulu vati” by column chromatography. Characterization of isolated flavonoid fraction by FT-IR and GC-MS. To evaluate the IC50 concentration of isolated flavonoid fraction of Kanchanara guggulu vati by in- vitro cytotoxicity method (MTT assay). To determine the DNA fragmentation activity of isolated flavonoid fraction from “Kanchanara guggulu vati”.DISCUSSION: Breast cancer is malignant neoplasm of breast tissue. It is most commonly occurring cancer in females. It accounts for 23% of newly occurring cancer worldwide and represents 13.7% of all cancer death. In both developed and developing countries, breast cancer mortality and incidence rates are projected to rising continuously.[10] With increase in mortality rates among patients suffering from cancer with limited success being achieved in clinical therapies including radiation, chemotherapy, immune modulation and surgery in treating cancer patients, there arises a need for new way for cancer treatment.[95] Nature has given us a variety of useful medication to cure number of diseases. The role of natural products as a source of remedies for diseases can be dated back to 1500 BC. It has been estimated that 60% of the approved drugs used for treating cancer are derived from natural sources. After long folk practices, many Indian medicines have been screened and they are used for treating and preventing various chronic disorders like cardiovascular diseases and cancer. Ayurvedic medicines were found to be able to cure diseases better, which were previously not amenable to Western practices. Traditional Indian medicine with its evolution through centuries has always fascinated practitioners and researchers for its applications in a cancer treatment on a scientifically proven research background. Herbal drugs consisting of multiple herb each possessing tremendous potential for a cancer cure are commonly used in Ayurveda. [1] Kanchanara guggulu vati is an ayurvedic polyherbal formulation which consisting of twelve herbs and it is extensively used for the treatment of hypothyroidism and obesity, PCOS, tumor, skin diseases and also used as immune system booster, appetite stimulant, and detoxifier.[19] The phytochemical analysis of methanolic crude extract from Kanchanara guggulu vati revealed that the presence of flavonoids, phenols, tannins, and alkaloids.CONCLUSION: The phyto chemical investigation revealed the presence of flavonoids, phenolic acids, alkaloids, fixed oils& fatty acids, and steroids, carbohydrates and saponins.  MTT assay was carried out for methanolic crude extract of Kanchanara guggulu, which inhibited 50% of cell growth at the concentration of 55.26μg/ml. The flavonoid fraction was isolated by column chromatography and it was characterized by FT-IR and GC-MS. The isolated flavonoid fraction was subjected to MTT assay, which inhibited 50% of cell growth at the concentration of 38.68μg/ml. DNA fragmentation was observed in flavonoid fraction treated cells, which confirmed the ability of Kanchanara guggulu induced apoptotic cell death. The cytotoxicity activity, apoptotic studies which were carried out in extract of Kanchanara guggulu using in-vitro methods can be confirmed with in-vivo animal models for the future perspective.