EMIT 法によるミコフェノール酸血中濃度測定とHPLC 法との比較による血中濃度評価

EMIT,a homogeneous enzyme immunoassay for the quantitative analysis of mycophenolic acid (MPA) in human plasma,was evaluated in comparison with high-performance liquid chromatography (HPLC).For EMIT,coefficients of variation (CV) for within-run measurements (1~12μg/mL,n=20)varied from 4.07% to 5.16% and CVs for between-day measurements (1~12μg/mL,n=20)ranged from 2.23% to 5.63%.The lower limit of detection was 0.20μg/mL.When MPA plasma concentrations were simultaneously measured using EMIT and HPLC,there was a good linear relationship between the 2 assay methods showing a high degree of correlation : EMIT=1.13×HPLC+0.164 ; r2=0.967,51 points in 16 liver transplant recipients,EMIT=1.10×HPLC+0.305 ; r2=0.948,55 points in 15 renal transplant recipients and EMIT=1.06×HPLC-0.014 ; r2=0.887,300 points in 8 islet transplant recipients.These results indicate that EMIT is a convenient means of measuring MPA in plasma.