The Effect and Mechanism of Component Compatibility of Rhynchophylla Total Alkaloids and Sinapine Cyanide Sulfonate on Change of Vascular Endothelial Cells

Aim To study the effect and mechanism of rhynchophylla total alkaloids,sinapine cyanide sulfonate and its component compatibility on change of vascular endothelial cells induced by the tumor necrosis factor-α(TNF-α).Methods Rat vascular endothelial cells were cultured in vitro,and vascular endothelial cell injury model was established by TNF-α.Then changes of cell morphology were observed before and after treatment;intercellular adhesion molecule-1(ICAM-1),vascular cell adhesion molecule-1(VCAM-1) and rat endothelin-1(ET-1) were determined by Elisa method,mRNA of ICAM-1,VCAM-1 and ET-1 by RT-PCR method and the level change of endothelium-derived relaxing factor(NO) by nitrate reductase method.Results The vascular endothelial cell injury model could be established in vitro successfully by TNF-α(5 μg/L).Compared with TNF-α group,treated groups could improve vascular endothelial cell morphology,lower the secretion of ICAM-1,VCAM-1 and ET-1.Compared with rhynchophylla total alkaloids group and sinapine cyanide sulfonate group,the component compatibility group was better on heightening the secretion of NO,down-regulating the expression of ICAM-1 mRNA(P0.05).On down-regulating the expression of mRNA of VCAM-1and ET-1,the rhynchophylla total alkaloids group and the component compatibility group were better than the sinapine cyanide sulfonate group(P0.05).Conclusion The component compatibility of rhynchophylla total alkaloids and sinapine cyanide sulfonate has the function of protecting rat vascular endothelial cell injury induced by TNF-α.