Computerized video time lapse study of cell cycle delay and arrest, mitotic catastrophe, apoptosis and clonogenic survival in irradiated 14-3-3σ and CDKN1A (p21) knockout cell lines

Abstract Chu, K., Teele, N., Dewey, M. W., Albright, N. and Dewey, W. C. Computerized Video Time Lapse Study of Cell Cycle Delay and Arrest, Mitotic Catastrophe, Apoptosis and Clonogenic Survival in Irradiated 14-3-3σ and CDKN1A (p21) Knockout Cell Lines. Radiat. Res. 162, 270–286 (2004). Computerized video time lapse (CVTL) microscopy was used to observe cellular events induced by ionizing radiation (10–12 Gy) in nonclonogenic cells of the wild-type HCT116 colorectal carcinoma cell line and its three isogenic derivative lines in which p21 (CDKN1A), 14-3-3σ or both checkpoint genes (double-knockout) had been knocked out. Cells that fused after mitosis or failed to complete mitosis were classified together as cells that underwent mitotic catastrophe. Seventeen percent of the wild-type cells and 34–47% of the knockout cells underwent mitotic catastrophe to enter generation 1 with a 4N content of DNA, i.e., the same DNA content as irradiated cells arrested in G2 at the end of generation 0. Radiation caused a...