Effects of bisphenol-A on expression of estrogen receptor and level of 17 β-eatradiol in vivo of female Carassius auratus

The effects of bisphenol-A(BPA) on female Carassius auratus were investigated in vivo.Fishes were given intraperitoneal injection with the dose of 1 mg/kg,50 mg/kg,100 mg/kg of BPA.The expression of estrogen receptor alpha(ERα) mRNA in liver and the level of E2 in serum were checked in 24 h and 48 h after injection.The results showed that there was no significant increase of expression of ERα mRNA both in 1 mg/kg and 50 mg/kg of BPA groups;in 100 mg/kg of BPA group,the expression of ERα mRNA increased significantly both in 24 h and 48 h(P0.05).The level of E2 decreased significantly in 1 mg/kg of BPA group in 24 h,then went up to the normal level in 48 h.In 50 mg/kg and 100 mg/kg of BPA groups,the levels of E2 increased significantly in 24 h and maintained at the high level in 48 h.These results indicated that BPA had estrogen-like effects.100 mg/kg of BPA can up-regulate the expression of ERα mRNA in liver and the level of E2 in serum of female C.auratus.