Influência da concentração de BAP e AG3 no desenvolvimento in vitro de mandioquinha-salsa

Two experiments were carried out to evaluate the effect of BAP and GA3 concentrations on the in vitro arracacha development. The concentrations tested were 0.0; 0.2 and 0.4 mg L-1 of BAP and 0.0; 0.125 and 0.250 mg L-1 of GA3 in the shoot tips with about 2 mm of arracacha, cultivars Amarela de Senador Amaral and Amarela Comum. The interaction of cultivars x BAP concentration was not significant for any one of the evaluated variables (P>0,0617). The increase in the BAP concentration reduced the callus formation; however, promoted larger reduction in the shoot development. The best BAP concentration was around 0.3 mg L-1, with relatively reduced callus diameter and good development of shoot, with a mean of 4.5 shoots and a height of 43 mm. The interaction of cultivars x GA3 concentration was not significant for any one of the studied variables (P>0,4310). The increase in the GA3 concentration promoted the improvement in the mean and maximum height of the shoots and in callus size. More shoots were observed for Amarela Comum cultivar in both trials.