Direct high-performance liquid chromatographic resolution of dihydropyridine enantiomers

Abstract A direct high-performance liquid chromatographic enantioseparation on a chiral stationary phase using a protein as chiral selector was achieved for some dihydropyridines with different structural groups (amine, amide, acid and hydroxyl groups). The protein involved was α 1 -acid glycoprotein and the work was mainly carried out with an EnantioPac column. An improved version of this chiral column (Chiral-AGP) was compared with the previous one and gave interesting results.