TGF-β Prevents the Denervation-induced Reduction of Bone Formation and Promotes the Bone Regeneration through Inhibiting Ubiquitin-proteosome Pathway

Background: Transforming growth factor beta (TGF-β) can stimulates for osteogenesis as a multifunctional protein. This study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. Materials & Methods: The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in skeletal tissue by real-time PCR. Results: RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (Runt-related transcription factor 2), OC and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. Conclusion: TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteosome pathway at least partially.