A Chromatographic Strip for Rapid Semi-quantitative Detection of H5 Subtype Avian Influenza Viruses in Poultry

An immunochromatographic strip was developed for rapid semi-quantitative of the H5 subtype of avian influenza viruses (H5 AIVs) in poultry. A specific monoclonal antibody 1C4 for H5 AIV hemagglutinin (HA) was produced and conjugated with colloidal gold as the detecting probes for the immunochromatographic strips, and a polyclonal antibody against H5N1 was used to firm the test line. Different from the traditional strips, four test lines with a same capture complex of different concentrations were designed on the nitrocellulose membrane. By contrast with the HA tests and the simulated infection experiment, the immune strip was proved to be specific for the detection of H5 AIV within 10 min and had the character to reflect the viruses infection degree in different organs of the infected poultry. It should be useful for diagnosing and semi-quantitative analysis of the H5 subtype of AIV.