PCR primers for sex identification of nyctereutes procyonoides ussuriensis matschie and an identification method thereof

PCR primers for sex identification of nyctereutes procyonoides ussuriensis matschie are disclosed. The nucleotide sequences of the PCR primers are shown as a sequence 1 and a sequence 2 in a nucleotide sequence table. An identification method for sex identification of nyctereutes procyonoides ussuriensis matschie by using the PCR primers is also provided. The method includes performing PCR amplification by adopting total DNA of nyctereutes procyonoides ussuriensis matschie to be identified as a template and by using the PCR primers, and determining sex of a nyctereutes procyonoides ussuriensis matschie individual by combining the PCR amplification and an agarose gel electrophoresis technique. The PCR primers and the method are used for sex identification of the nyctereutes procyonoides ussuriensis matschie, and are rapid, simple, convenient and accurate in result.