Effect of adenine sulfate (AdSO4) on the in vitro evolution of white clover variety (Trifolium repens L.).

We tested the effect of adenine sulfate on the in vitro behavior of the explants from Trifolium repens L bud. After 40 days of in vitro culture observations were made on the regeneration percentage of the explant, on the number of generated neoplantlets and on their length, on the differentiation of the root system and last, but not least on the formation of nodules on the roots. Adenine sulfate in the medium has generated the formation of a greater number of neoplantlets, when it is associated in the medium with cytokinine. The experiment has shown the fact that from cytokinine, benzyl adenine (BA) with adenine sulfate in low dose of 1 mg/l has stimulated a good regeneration percentage (80%), with a balanced number of plants (about 8 plants/explant), but also the formation of nodules along the root system, much thickened as in the absence of adenine sulfate. The best variant proved to be V6 (MS+1.0mg/l Z +0.1mg/lAIA+40mg/lAdSO4) containing zeatine which stimulated the parameters under the most balanced values. Noteworthy is the effect of adenine sulfate on the callus and nodules differentiation, in the presence of 1mg/l dose of zeatine or benzyl