Microbial dynamics during the composting process

Microbial dynamics of microbial community during the composting process was investigated with the methods of denaturing gradient gel electrophoresis(DGGE) and plating.The results showed that the most of microorganism count was bacterium,the second was actinomycete and the least was fungi.At the same time, the count of the thermophilic microorganism was always less than that of the mesophilic during the composting process.The count of mesophilic microorganism at later stage was less than that at the initial stage.However,the count of thermophilic antinomycete and fungi at later stage were more than that at initial stage,and the count of thermophilic bacterium was stable throughout the composting process.The bands pattern of DGGE and 16S rDNA analyses indicated that bacterial succession was presented during the composting process.The genera of Bdellovibrio,Clostridia bacterium and Bacillus were dominant species at initial stage(before the first 15 days),and Beta proteobacterium,Petrobacter succinimandens,Nitrospirae bacterium and Paenibacillus were dominant species at middle and later stage.Moreover,Clostridium was found throughout the process.