Bergenin: Review of The Methods of Determination and Quantification

Studies have shown that Bergenin has various pharmacological functions, such as Anti-lithiatic, Anti-oxidant , anti-inflammatory, Anti-bacterial, Anti-viral, Anti-cancer, Anti-cholinesterase, Anti-diabetic, Hypolipidemic, Anti-tussive, Hepatoprotective and Immunomodulatory . Bergenin is found in the Saxifragaceae, Euphorbiaceae, Myrsinaceae, Dipterocarpaceae, and Fabaceae genera and a range of beneficial effects on human health. The number of bioactive compounds in various bergenia species must be determined in order to achieve the best quality raw material for herbal preparations. As a result, developing analytical methods to investigate the pharmacokinetics and activity mechanisms of berberine alkaloids, as well as a more systematic analysis of berberine alkaloids, is worthwhile. Thin-layer chromatography (TLC), high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), mass spectroscopy (MS), and other analytical techniques are used to identify Bergenin. Since plant extracts have complex structures, isolating and identifying active compounds is a complicated, time-consuming, and costly. This present review summarizes analytical method for identification and quantification of Bergenin.