FRET Efficiency Measurement with a Low-Cost Frequency Domain Fluorescence Lifetime Imaging Microscope

John Paul Dumas,James Jiang,Evan M. Gates,Brenton D. Hoffman,Mark C. Pierce,Nada N. Boustany

FRET Efficiency Measurement with a Low-Cost Frequency Domain Fluorescence Lifetime Imaging Microscope

2020

John Paul Dumas
James Jiang
Evan M. Gates
Brenton D. Hoffman
Mark C. Pierce
Nada N. Boustany

We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.

Keywords:

Microscope
Phase (waves)
Fluorescence-lifetime imaging microscopy
Förster resonance energy transfer
Optics
Frequency modulation
Frequency domain
Materials science
Vinculin
Calibration curve
Fast Fourier transform

Correction
Source
Cite
Save
Machine Reading By IdeaReader

References

Citations