Enzyme Fractionation by Means of Large- Scale Continuous- Flow Electrophoresis

Large- scale, microglass bead, continuous- flow electrophoresis was used to separateand collect protein fractions from a single serum sample. These fractions were assayed for a multiplicity of enzyme activities. Relatively good recovery was obtained with lactic acid dehydrogenase, leucine aminopeptidase, and phosphohexoseisomerase. Artifactually high recoveries were observed with aldotase and isocitric acid dehydrogenase. The increase observed in aldolase activity may be due to a pH effect.