Development and evaluation of armored RNA-based standards for quantification of BCR-ABL1 p210/p190 fusion gene transcripts

Background Standards play an important role in detection of the BCR‐ABL1 fusion gene (FG) transcript. However, the standards widely used in laboratories are mainly based on plasmids or cDNA, which cannot accurately reflect the process of RNA extraction and cDNA synthesis. Therefore, we aimed to develop armored RNA‐based standards for p210 and p190 BCR‐ABL1 FG transcripts’ quantification.