Biyoimpedans İğne Probu ile Parçacık Boyut Tespiti

Objectives: The aim of this study was to test the Bioimpedance probe needle on in-vitro tissue phantom. Study Design: Bioimpedance probe needle that was connected to a bioimpedance measurement device to record spectra in the kHz range. The probe consists of an 18Gauge 1.2x89 mm spinal needle. The probe was used for obtaining impedance information about the polystyrene microspheres which were in different diameters; 1, 2 and 4 micrometers. The current was transferred to the phantom and conductivity information was obtained by the probe. Results: Bioimpedance probe needle allows the discrimination of different particle sizes based on their specific signatures. The sensitivity of the probe to particle size in tissue phantom was tested during the study. Spectroscopic data were evaluated with resistance values at 50 kHz and the information about the particle size in the tissue phantom was obtained. Bioimpedance spectra results which were obtained from polystyrene microspheres showed that the resistance values were increasing while the size of the particle was enlarging. Conclusion: Due to the highly accurate differentiation of particle size in the tissue phantom, our probe has the potential to be used in the rapid detection of Escherichia coli or Acinetobacter baumannii which have different sized microorganisms in the tapped cerebrospinal fluid without being cultured in real time. In addition, it is possible for the probe to perform cell counting and differentiation in cell culture studies.