The anti-inflammation effect of hydrogen saline on paw oedema.

Objective:To study the anti-inflammation effect of hydrogen saline on carrageenan induced paw oedema.Methods:The severity of inflammation damage was evaluated by paw volume measurement and inflammatory cells infiltration.TNF-α in supernatants was evaluated by ELISA.RT-PCR was used to characterize the mRNA expression of TNF-α in RAW264.7 macrophages.Results:Hydrogen saline showed dose dependently down-regulated carrageenan-induced paw swelling,compared with the vehicle control group,which received an equal volume of vehicle only(saline).The treatment of animals with hydrogen saline produced a significant decrease in the number of infiltration neutrophils in the inflammatory paw.Supernantant TNF-α level from activated macrophages(collected 4h after LPS stimulation) treated with saline were significantly higher than that treated with hydrogen saline.TNF-α mRNA of activated macrophages treated with hydrogen saline was lower than that treated with saline.Conclusions:Our findings indicate that the hydrogen saline had the effect of anti-inflammation in both the paw oedema models and LPS-activated macrophages.The possible mechanism may work by reducing oOH and ONOO-.So we could conclude that hydrogen saline may be a potential candidate for the therapy of inflam-matory diseases,which is more convenient to be administered than inhaling hydrogen.