THE COMPARISON OF PREDOMINANT ORAL MICRO-FLORA IN SUBJECTS WITH AND WITHOUT COMPLETE DENTURE REFERRED TO YAZD DENTISTRY DEPARTMENT

Introduction: Human oral cavity consist a mass of microorganisms, which may altered by application the complete removable denture in edentulous patients. The aim of present study was to compare the dominant oral micro-flora between edentulous denture users with dentate elderlyThe aim of the present study was to compare the dominant oral micro-flora between edentulous denture users with dentate elderly. Materials and Method: In the current analytical, cross-sectional study, 30 edentulous patients with complete removable denture and 30 dentate subjects, who had been admitted for non-prosthetic treatments, were randomly selected in Yazd dentistry department. Their oral cavity samples were obtained using sterile cotton-tip swabs for direct smear analysis and cultivation on the selective fungal and bacterial media. Isolated predominant bacteria and fungi were enumerated and identified by microbiological differential diagnosis tests. The data were analyzed with SPSS software with T test and the differences were considered statistically significant when p<0.05. Results: The non-aureus staphylococcus and alpha-hemolytic streptococci showed the highest positive culture among the isolated microorganisms in both groups whereas beta hemolytic streptococci showed the least percentage of isolated microorganisms in both groups. The higher density of non-aureus Staphylococci, α-hemolitic Streptococci, Gram negative cocobasillus, non-pathogenic Neisseria, Candida and Corynebacterium were recovered from oral samples of denture users in comparison with dentate subjects (P= 0.0001). A statistically significant correlation between the number of isolated microorganisms and the duration of denture utilization in denture users was also seen (P=0.013). Conclusion: The results of the present study showed that complete denture can act as a predisposal factor in the overgrowth of several oral micro-flora particularly Candida, non-aureus Staphylococci, α-hemolytic streptococci, gram negative cocobacillus, non-pathogenic Neisseria, and Corynebacterium, which emphasized the users denture