Efficiency of Rabies Isolation by Using Mouse Neuroblastoma Cells Compare with Mouse Inoculation

Rabies is an infectious agent in mammal which causes a fatal disease. In present several countries have an attempt to use cell culture for diagnosis and confirmation of rabies. In this study, we developed the method for isolation of rabies virus in cell culture and evaluated its efficiency in comparison with mouse inoculation test (MIT). The result revealed that the cell isolation method has 100% sensitivity, specificity and efficiency, respectively when compared with mouse inoculation test method. From this study, the isolation and confirmation of rabies can be considered to substitute the cell culture method for the mouse inoculation test method. Key word : Rabies, cell isolation, mouse inoculation, mouse neuroblastoma