New glycolipid inhibitors of Myt1 kinase

A crude extract of a marine alga showed activity against the enzyme Myt1 kinase. Bioassay-directed fractionation led to the isolation of two bioactive glycoglycerolipids. Lipid 1 was identified as sn-1,2-dipalmityl-3-(N-palmityl-6-deoxy-6-amino-a-D-glucosyl) glycerol and lipid2 as sn-1-palmityl-2-myristyl-3-(N-stearyl-6-deoxy-6-aminoglucosyl)glycerol. Compounds 1 and2 had IC50 values of 0.12 and 0.43 mg/mL, respectively, in the Myt1 kinase inhibitory bioassay, and were inactive against Akt and Chk1 kinases. q 2004 Elsevier Ltd. All rights reserved.