Inhibition of PI3K improves contractility in alpha1-adrenergically stimulated myocardium

1. Abstract 2. Introduction 3. Methods and materials 3.1. Isolation and primary culture of rabbit ventricular myocytes 3.2. Single myocyte shortening and intracellular Ca measurements 3.3. Preparation and contractile testing of intact muscle strips 3.4. Myofilament Ca responsiveness in semi-skinned muscle fibres 3.5. Protein expression and phosphorylation level 3.6. Statistical analysis 4. Results 4.1. Effect of PI3K inhibition on alpha1-adrenergically stimulated single myocytes 4.2. Effect of PI3K inhibition on alpha1-adrenergically stimulated intact muscle strips 4.3. Effect of PI3K inhibition on Ca transients 4.4. Effect of PI3K inhibition on Ca sensitivity in semi-skinned fibres 4.5. Effect of PI3K inhibition on protein expression and phosphorylation level 5. Discussion 6. Acknowledgements 7. References