Kit used for detecting Tim-3, and application method thereof

The invention belongs to the technical field of biotechnology, and discloses a kit used for detecting Tim-3, and an application method thereof. The invention also discloses monoclonal antibodies used for detecting Tim-3, or fragments of the monoclonal antibodies; the monoclonal antibodies or the fragments comprise light chain CDR1, light chain CDR2, light chain CDR3, heavy chain CDR1, heavy chain CDR2, and heavy chain CDR3; amino acid sequences of light chain CDR1, light chain CDR2, light chain CDR3, heavy chain CDR1, heavy chain CDR2, and heavy chain CDR3 are represented by SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, or SEQ ID No.6 respectively. The monoclonal antibodies are capable of realizing specific binding with human Tim-3; the kit which is prepared from the monoclonal antibodies and is used for detecting human Tim-3 possesses relatively high sensitivity, and can be used for detecting of human Tim-3 with low concentration.