Quantitative FRET measurement of the interaction of A1 adenosine receptors and A2A adenosine receptors in living cell

Adenosine plays important roles in the pain signal transduction by activating adenosine receptors of two subtypes of A1 and A2A. In this study, FRET system based independent emission -spectral spectral spectral unmixing method (Iem-spFRET) was set up and used to measure the energy transfer from A1R to A2AR. The energy transfer efficiency calculated by Iem-spFRET is about 17.44%. All the above date and results demonstrate that FRET with special designed fluorescence proteins could be used to investigate the interaction between adenosine receptors.