Comparative Study of Chronic Kidney Disease Stages by GFR Derived from CKD-EPI Formula Using Enzymatic and Jaffe’s Kinetic Methods in Diabetic Patients Type 2

The purpose of this study was to compare the eGFR with CKD-EPI from serum creatinine determined by Jaffe’s kinetic method (Cr Jaf ) and enzymatic method (Cr). The study  enz  was performed in 107 type 2 diabetic patients, 49 males and 58 females with mean age of 64 years recruited from diabetic clinic at Thungsong Hospital, Nakhon Si Thammarat Province. Highly correlated of Crand Cr Jaf in all subjects was found (r = 0.936, p < 0.001). However, enz  Cr was significantly lower compared to Cr Jaf  (1.31 ± 0.90 mg/dL and 1.41 ± 0.87 mg/dL,  enz  respectively, p < 0.001). Crin subjects with eGFR ≥ 60 mL/min/1.73 m 2  (n = 58) was lower enz  than Cr Jaf  significantly (0.83 ± 0.20 mg/dL and 0.97 ± 0.22 mg/dL, espectively, p < 0.001), whereas the difference between Cr and Cr Jaf  was not found in subjects with eGFR < 60 mL/ enz  min/1.73 m 2  (n = 49), (1.90 ± 1.50 mg/dL and 1.94 ± 1.05 mg/dL, respectively, p = 0.117). eGFR-Cr was significantly higher than eGFR-Cr Jaf  in all samples (65 ± 30 and 58 ± 25 mL/ enz  min/1.73 m 2 , respectively, p < 0.001) and those with eGFR ≥ 60 mL/min/1.73 m 2  (89 ± 16 and 77 ± 15 mL/min/1.73 m 2 , respectively, p < 0.001). The number of subjects with eGFR-Cr and  enz  eGFR-Cr Jaf ≥ 60 mL/min/1.73 m 2  were 59 and 51, and those with eGFR-Cr and eGFR-Cr Jaf  enz  < 60 mL/min/1.73 m 2  were 48 and 56, respectively. The same number of subjects with stage 4 and stage 5 CKD of eGFR-Crand eGFR-Cr Jaf were 11 and 5, respectively. Therefore, using  enz eGFR to differentiate CKD stages with creatinine determined by enzymatic method may not be superior to those with Jaffe’s kinetic method in type 2 diabetic patients.