Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique

Objective To explore the appropriate amount of template DNA for Sinofiler Kit.Methods The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.Results It was shown that 1.29-1.51 ng of template DNA in 12.5 μL reaction volume was optimal for STR genotyping with Sinofiler Kit.Conclusion Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.