Application of high-performance anion-exchange chromatography with pulsed amperometric detection to sugar analysis in citrus juices

A high-performance anion-exchange chromatographic method that uses an alkaline mobile phase on a pellicular quaternary amine resin column at ambient temperature, coupled with pulsed amperometric detection, has been applied to the analysis of the three major sugars: glucose, fructose, and sucrose. Data were compared with those obtained from independent analysis by HPLC on an amino-bonded silica column with refractive index detection. Limits of detection were estimated to be 45 ng for glucose, 60 ng for fructose, and 300 ng for sucrose