Genetic Mapping of Seed and Nodule Protein Markers in Diploid Alfalfa (Medicago sativa)

The genetic map of diploid alfalfa was generated based on the genetic analyses of genotypes of markers collected for the individuals of an F2 population originating from the cross of two diploid alfalfa subspecies, M. sativa ssp. quasifalcata and M. sativa ssp. coerulea (Kiss et al. 1993). The constructed linkage map composed of eight linkage groups which contain more than 1800 morphological, allozyme, and DNA-based markers, of which more than 300 represent loci with known function or sequences in the genome (Kalo et al. 2000). The markers cover approximately 760cM genetic distance. The average correlation between the physical and the genetic distance is about 1000kb/cM. This genetic system was used to map protein markers originating from nodule and seed tissue.