Zuverlässigkeit der Pollenanalyse von Honig : Bestimmung der Pollenhäufigkeit

1996 
The legal conformity of the declaration of type or origin of honey can be checked by its pollen distribution. In the working group,,pollenworkshop 14 collaborative trials of varying difficulties were carried out to determine the relative amounts of 3-6 pollen types in blossom honey. The experimental procedure followed those described in the article,,Methods of Mellisopalynology, International Commission for Bee Botany of IUBS. The evaluation of the results from light microscopy studies in the levels of 100, 300, 500, 1000 pollens was carried out using the,,Amtliche Sammlung von Untersuchungsverfahren, 35 LMBG. The decrease of the scattering in the analytical results with increasing pollen counts depends of the difficulty in determining the honey species. In the count range used in this study the decrease of the reproducibility with increasing number of pollens counted can be described by a power law. This also applies to comparability, except for values of honey with pollen species that are hard to differentiate. This method is well suited to quantify the fraction of defined pollen species. For the classification into frequency classes at least 300 pollen have to be counted, for more exact statements of the relative amounts 500-1000 pollen counts are necessary, depending on the stabilisation of the values.
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