Molecular markers for genic male sterility in Chinese cabbage

The gms gene of Chinese cabbage (Brassica campestris ssp. chinensis) conferring a recessivegenic male sterility was mapped with AFLPmarkers. Four markers were found to betightly linked to the gene with a distanceof <1 cM using a mapping population of327 individuals. The AFLPs were cloned andsequenced. Sequence derived primers yieldedclearly distinguishable PCR fragmentsrepresenting sequences tightly linked tothe gms gene. The sequence taggedsite (STS) markers can be used for markerassisted selection of male sterile plantsamong segregating populations.