A study of relations among PKC membrane translocation,activity changes and expression of TNF-alpha in rat monocytes exposed to hypoxia

Objective To investigate the role of PKC membrane translocation,activity changes in the expression of TNF-α in rat peripheral blood monouclear cells(PBMCs) exposed to hypoxia,and to explore the relationship between hypoxia and system inflammation response syndrome(SIRS).Methods Purified rat PBMCs were exposed to hypoxia(3%O2,5%CO2,92%N2) for 0,1,3,6,9,12,24h.The PKC membrane translocation was assayed by immuno-precipitation and SDS-PAGE.The PKC activity was assayed by PKC kit.The expression of TNF-α was detected by reverse transcriptase PCR(RT-PCR).The dependability among above indexes was analysis by SPSS10.0.Results The PKCα membrane translocation and activity increased significantly during 1-9h of hypoxia(P0.01).There were no significant changes of PKCe membrane translocation and activity during 1-24h of hypoxia.There were significant positive correlations among the PKCα membrane translocation,activities of PKCα and the expression of TNF-α(P0.01-0.05).Conclusion Hypoxia enhances the PKCα membrane translocation,the activities of PKCα and up-regulates the expression of TNF-α in mRNA subsequently.These changes might be one of the mechanisms of a mass of pro-inflammatory factors existing persistently in blood plasma in acute respiratory distress syndrome(ARDS) patients.