Use of radioisotope technics for determination of thyroid hormones. Research on blood and urine

: At first the AA. present the recent progresses reached in the Endocrinology diagnostic with the aid of the radioisotopic techniques; successively they refer about the principles regulating the chemical reactions used in the dosage of the single hormones and about the use of index interesting the clinical practice. The AA. compare the methods of dosage used in the kits of various firms outlining the problems concerning the technological times, the operations number, the contamination risk. They performed several control tests on the T3 RIA, recently introduced into the clinical diagnostic, on the calibration curve, on the repeatibility of the results, on the recovery tests, on the sensitivity of the tarature curve, showing the normal and abnormal values obtained in their hospital. They refer moreover on the results obtained dosing together on the same samples T4, TBC, ETR, T3, RIA, TSH and on the clinical utility of the single tests. Several dosages of T3 and of T4 were effected on urine to have informations on the urinary excretion levels of the thyroid hormones in normal and abnormal subjects. At last they hypothesize the use of the hormonal urinary values and the plasmatic ones for calculating an ormonal thyroid clearance.