Characterization of onion genotypes by use of RAPD markers
2012
In order to estimate, at the molecular level, the divergence of parental
lines that were used in diallel crossbreeding for production of superior
offspring (F1 generation hybrids) at the Institute for Vegetable Crops, the
molecular analysis using five RAPD markers for five pairs of parents has been
performed. It gives an insight into their genetic polymorphism and the
possibility of their further use in breeding programs. Information from this
research has pioneered the application of molecular markers of onion in
Serbia. Analyses were performed using the RAPD primers, which in previous
studies established a high degree of polymorphism. In all five cases there
was a corresponding amplification of DNA segments. From totally 50 bands
analyzed, the length of fragments ranged from 500 to 3000 bp. Number of
polymorphic band per example was 8 to 13. In our research at the level of the
analyzed primers, a high degree of polymorphism between analyzed genotypes
has been found. Based on UPGMA dendogram, analyzed genotypes were divided
into two main clusters and two subclusters.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
11
References
8
Citations
NaN
KQI