Chemical analysis of cigarette smoke particulate generated in the MSB-01 in vitro whole smoke exposure system

Cigarette mainstream smoke (MS) is a dynamic aerosol consisting of a gas–vapor phase and a particulate phase. In recent years, novel in vitro whole smoke exposure systems have been developed to expose cells directly to whole MS. One such system is the Burghart Mimic Smoker-01 (MSB-01). Our previous data using the MSB-01 indicated that a 50 ± 10% loss of particulate matter occurred prior to MS delivery into the exposure chamber. Additionally, a change in aerosol particle diameter was also measured, suggesting that the chemical composition of MS might be changing within the system. In this study, we have expanded on our previous work and compared the particulate phase chemical composition of undiluted and diluted MS generated by the instrument and that of the MS delivered into the exposure chamber. The average percent delivery of cigarette smoke condensate (CSC) detected for all the measured chemical constituents was 35 ± 13% for undiluted MS and 23 ± 8% for 1:1 diluted MS. The data also indicate that under...