Determination of Content of VB2 Tablet with Fluorescence Fiber-optic Chemical Sensor

[Objective]Using fiber optic chemical sensor based fluorescence method to accurately determine the content of VB2tablets.[Method]VB2tablets were dissolved in aqueous solution and the detection system of fiber optic chemical sensor based fluorescence method was used to measure the spectrum of the solution and to quantitative analyze the content of VB2.[Result]With this method the maximum emission wavelength of VB2was 533 nm.The mass concentration was3.2×10-5-8×10-4mg/ml,which had good linear relationship with fluorescence intensity.The detection line was 1.55×10-8mg/ml and within-day precision was 0.19%while day to day precision was 1.2%,and the recovery was 97.9%-105.1%.[Conclusion]The degree of accuracy was high and the detection line was low when this method was used to determine the content of VB2tablets,and the results were satisfactory.