SIMULTANEOUS ESTIMATION OF ATAZANAVIR AND RITONAVIR IN TABLET DOSAGE FORM BY HPTLC METHOD

A simple, sensitive and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of atazanavir and ritonavir in pharmaceutical formulations. The chromatographic development was carried out on HPTLC plates pre-coated with silica gel 60G F254 using a mixture of toluene: ethyl acetate: 0.1% formic acid in the ratio of 6.0:4.0:1.0 v/v as mobile phase. The calibration curve was found to be linear over the concentration range of 150-900 ng/spot for ATV and 50-300 ng/spot for RTV with a regression coefficient for both analytes were greater than 0.999. The %RSD values for intra-day and inter-day variation were not more than 2.0. The method has demonstrated high sensitivity and specificity. The method is new, simple and economic for routine estimation of atazanavir and ritonavir in bulk and pharmaceutical formulation to help the industries as well as researchers for their sensitive determination of atazanavir and ritonavir rapidly at low cost in routine analysis.