Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus

Abstract Pigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a Taq Man-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/μl. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the transmission of torque teno virus in pigeons.