Giant panda semen freezing and preserving fluid and preparation method thereof

The invention discloses a giant panda semen freezing and preserving fluid and a preparation method thereof. The giant panda semen freezing and preserving fluid disclosed by the invention is prepared from glucose, levulose, trisodium citrate, yolk, glycerin and distilled water. The preparation method comprises the following steps of: firstly, dissolving solid ingredients; then, adding liquid ingredients; and fully mixing uniformly to obtain a finished product. By using the freezing and preserving fluid disclosed by the invention for freezing and preserving giant panda semen, the activity of the unfrozen semen accords with the requirements of artificial fertilization, the average activity is greater than or equal to 58%, and the maximum activity is 65%. Compared with the American TEST preserving fluid, the giant panda semen freezing and preserving fluid has the advantages that the required reagents and raw materials are convenient to acquire and are low in price, the cost for preparing 100mL of the freezing and preserving fluid is about 50 yuan, the preparation method is simple and convenient, the fluid can be prepared and then used on the site, the instant preservation of the semen can be realized, and the frozen semen can be preserved for more than 50 years in liquid nitrogen. The giant panda semen freezing and preserving fluid provides the semen which accords with the requirements of artificial fertilization of giant pandas, greatly lowers the cost for artificially breeding giant pandas and preserving semen for a long time, and has important significance for improving the reproductive performance of giant pandas and enlarging the population quantity of giant pandas.