reverse CAMP test. test for Clostridium perfringens: New presumptive identification

at either 37or45°C. Resulting blackcolo-nies weregramstained andidentified as C.per-fringensif theyproduced a typical double zoneofbeta-hemolysisonTrypticasesoysheepbloodagar (BBL) plates and gave appropriate reac-tions in supplemented nitrate-motiity medium(13) andlactose-gelatin medium(14). Ifequivo-cal reactions wereobtained in these media, fer-mentationproductsweredeterminedbygas-liq-uid chromatography (15) or additional charac-teristics weretested withtheBBL-Miniteksys-tem(19).Onestrainof