Simultaneous Determination of Urinary Uronic Acids and Saccharic Acids by Gas Chromatography

A simultaneous determination by gas chromatography of urinary uronic acids and saccharic acids is described. Uronic acids are converted into corresponding stable aldonic acids with sodium borohydride, and contaminative basic, aromatic and neutral substances are removed with columns of Amberlite CG-120, XAD-2 and CG-400. After trimethylsilylation of carboxyl and hydroxyl groups, these acids are separately determined by gas chromatography. By this method, D-glucuronic acid and D-glucaric acid were determined and moreover, free D-gluconic acid and alkali-labile glucuronides were analyzed from normal human urine.