Transparent Window Vibrational Probes for the Characterization of Proteins With High Structural and Temporal Resolution

Vibrational spectroscopy provides a direct route to the physicochemical characterization of molecules. While both IR and Raman spectroscopy have been used for decades to provide detailed characterizations of small molecules, similar studies with proteins are largely precluded due to spectral congestion. However, the vibrational spectra of proteins do include a “transparent window”, between ∼1800 and ∼2500 cm–1, and progress is now being made to develop site-specifically incorporated carbon–deuterium (C–D), cyano (CN), thiocyanate (SCN), and azide (N3) “transparent window vibrational probes” that absorb within this window and report on their environment to facilitate the characterization of proteins with small molecule-like detail. This Review opens with a brief discussion of the advantages and limitations of conventional vibrational spectroscopy and then discusses the strengths and weaknesses of the different transparent window vibrational probes, methods by which they may be site-specifically incorporate...