Dynamic thiol/disulphide homeostasis as oxidative stress marker in diabetic ketoacidosis.

BACKGROUND The aim of present study was to investigate the dynamic thiol/disulphide homeostasis as oxidative stress marker in diabetic ketoacidosis (DKA). METHOD A total of 77 participants consisting of 32 patients with DKA and 45 healthy volunteers were included in the study. Thiol/disulphide homeostasis (TDH) [total thiol-native thiol/disulphide changes] were measured in both groups (patient group and control group) using a brand novel method developed by Erel and Neselioglu. Half of the difference between total thiol and native thiol concentrations gives the amount of disulphide bond. RESULTS Total thiol, native thiol and disulphide levels in blood were found to be low. The levels of total thiol (p<0,001) and native thiol (p<0,001 ) were significantly lower in patients with DKA than in the control group. At the same time, the level of disulphide was non significantly lower in the patient group than the control group (p = 0.388 ). The level of IMA was higher in the patient group than the control group (p<0,001). CONCLUSIONS The total thiol ,native thiol and disulphide levels in DKA decrease in favor of oxidative stress.