Development of markers for shoot organogenesis in roses

Shoot organogenesis is an essential step for genetic engineering and the study of developmental processes in roses. Several adventitious shoot regeneration protocols have been established, but the number of responding cultivars is very limited. Furthermore, large differences in regeneration capacity between genotypes were reported rendering many cultivars as recalcitrant for shoot regeneration. Therefore, knowledge about the genetic complexity of the capacity for adventitious shoot regeneration and genes influencing this trait would be helpful for optimising regeneration protocols. Previously we analysed an association panel of 96 genotypes for in vitro shoot regeneration from petioles, and we detected a number of markers associated with the phenotypic traits. Here we present results of experiments to verify significantly associated markers in independent populations of garden roses using the KASP (Kompetitive Allele Specific PCR) technology.