Properties of neural crest-like cells differentiated from human embryonic stem cells.
2014
Neural crest cells (NCCs) derive early in vertebrate
ontogenesis from neural tube as a population of migratory cells
with exquisite differentiation potential. Abnormalities in NCC
behaviour are cause of debilitating diseases including cancers
and a spectrum of neurocristopathies. Thanks to their
multilineage differentiation capacity NCCs offer a cell source
for regenerative medicine. Both these aspects make NCC biology
an important issue to study, which can currently be addressed
using methodologies based on pluripotent stem cells. Here we
contributed to understanding the biology of human NCCs by
refining the protocol for differentiation/propagation of
NCC-like cells from human embryonic stem cells and by
characterizing the molecular and functional phenotype of such
cells. Most importantly, we improved formulation of media for
NCC culture, we found that poly-L-ornithine combined with
fibronectin provide good support for NCC growth, we unravelled
the tendency of cultured NCCs to maintain heterogeneity of
CD271 expression, and we showed that NCCs derived here possess
the capacity to react to BMP4 signals by dramatically
up-regulating MSX1, which is linked to odontogenesis.
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