Spectrofluorimetric determination of anthraquinone content in Morinda Officinalis How.

AIM:To develop the spectrofluorimetric method to determine the anthraquinone content of Morinda Officinalis How,and to discuss the relations of anthraquinone content of Morinda Officinalis in different metropolis preliminarily.METHODS:1,8-dihydroxyanthraquinone was reference substance.Take acetone as solvent agent,the fluorescent intensity of the solution was measured at 425/513 nm.RESULTS:The linear range was 247.5-2970 μg/L(r=0.9995).Free anthraquinone and combined anthraquinone content of Morinda Officinalis in different growing areas range were 29.26-69.95 μg/g,3714.59-5830.39 μg/g.CONCLUSION:This research for measuring anthraquinone content in Morinda Officinalis provides a kind of effectively dependable analytical method.Free anthraquinone and combined anthraquinone compositions of Morinda Officinalis in different metropolis have some differences.