Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.

An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a molecular mass of 37 kDa and a pI of 5.9. A possible role of the enzyme in flavor production during alcoholic fermentation is discussed.