detection of cytomegalovirus nucleic acid and mycoplasma nucleic acid in alveolar lavage fluid of 31 children with respiratory tract infection
1. Abstract
1.1 Objective: To investigate the infection of cytomegalovirus and Mycoplasma pneumoniae in alveolar lavage
fluid of children with respiratory tract infection. 1.2 Methods: A total of 31 children with respiratory tract infection
were enrolled in the Department of Pediatrics, the First Affiliated Hospital of
Anhui Medical University from May to August in 2017, and the cytomegalovirus
nucleic acid in the alveolar lavage fluid was detected by real-time fluorescent
polymerase chain reaction. Ribonucleic Acid (RNA) thermostatic amplification
technology was used to detect Mycoplasma
pneumoniae nucleic acid in the alveolar lavage fluid. 1.3 Results: The total detection rate of both pathogens was 64.52%,
the positive rate of cytomegalovirus nucleic acid was 38.71%, and the positive
rate of Mycoplasma pneumoniae was
25.81%. 1.4 Conclusion: Cytomegalovirus and Mycoplasma
pneumoniae have high infection rate in children with respiratory tract
infection. The combined detection of these two infection agents in alveolar
lavage fluid has important application value for clinical etiology and treatment 2. Keywords: Alveolar Lavage
Fluid; Cytomegalovirus Nucleic Acid; Mycoplasma
pneumoniae Nucleic Acid; Respiratory Tract
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