Effect of salicylhydroxamic acid (SHAM) and pyridoxine on bioactive compounds and enzyme activity of germinated wheat

This research was conducted in order to study the effect of priming on wheat seed (Triticum aestivum L.) by pyridoxine and salicylhydroxamic acid (SHAM) on germination and development characteristics as factorial design. Factors include pyridoxine with densities (witness), 0.02, 0.04 and 0.06 percent and SHAM was 0, 25 and 50 ppm. Germinated seeds were counted daily for eight days. Then the percent of germination, the length of the shoot, root and the root dry weight and also the amount of catalase and peroxidase activity were measured. The results showed that pyridoxine and SHAM affects on the percent of germination, the root dry weight, the length of the shoot and the root and the amount of catalase and peroxidase enzyme activity on the level of one percent (p>0.01) meaningfully. However, seed priming salicylhydroxamic acid led to increase of germination indexes and the reduction of the respective enzymes' activity such as peroxidase and catalase which is probably caused by the neutralization of free radicals. To sum up, the results showed that pre treatment seed by the pyridoxine with 0.06 percent an SHAM with 25 ppm had the best impact on germination indexes which can help the farmers in setting and producing suitable wheat plant.