USE OF CLADOPHORA GLOMERATA EXTRACT AGAINST MULTIDRUG RESISTANT BACTERIAL PATHOGENS

The antibacterial effect of the crude hot Chloroform extracts and purified fractions of Cladophoraglomerata (Linnaeus) Kutzing (Cladophoraceae) against multidrug resistant human pathogen were isolated from burn and bound. The test bacterial strains were Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris and Escherichia coli. Hot Chloroform extracts (128 and 256 mg/mL) of Cladophora glomerata inhibited growth of all the test organisms. Primary detection of active compounds showed that macroalgae (Cladophoraglomerata) containing flavonoids, alkaloids, phenols, saponins, Glycosides and tannins. Gas Chromatography-Mass Spectrometry was used to know the compounds which responsible of antibacterial activity and they were Salicylic acid was found to be a major compound (16.09%) followed by 6- Octadecenoic acid, (14.22%) and 10- Heptadecenoic acid (7.68%) and Tetradecanoic acid (7.29%) in addition to Dodecanoic acid (3.18%).These findings suggest the possibility of using the Cladophoraglomerata as a novel source of natural antimicrobial agents in pharmaceutical industries.